- Trade Union of Workers in Mines, Geology and Oil Industry
- OS ECHO (trade union of energy and chemical workers)
- Czech Metalworkers' Federation (OS KOVO)
- Trade Union of Building Workers of the Czech Republic (OS STAVBA)
- Universities Trade Union
- Trade Union of Workers in Woodworking Industry, Forestry and Management of Water Supplies
- Trade Union of Workers in Textile, Clothing and Leather Industry of Bohemia and Moravia
- Trade Union UNIOS (communal services)
- Independent Trade Union of Workers in the Food Industry and Allied Trades of Bohemia and Moravia
- Trade Union of Workers in Postal, Telecommunication and Newspaper Services in the Czech Republic
- Trade Union of Workers in Commerce
- Trade Union of State Bodies and Organisations
- Trade Union of Banking and Insurance Workers
- Trade Union of Health Service and Social Care in the Czech Republic
- Czech - Moravian Trade Union of Workers in Education
- Czech - Moravian Trade Union of Civilian Employees of the Army
- Czech Moravian Trade Union of Catering, Hotels and Tourism
- Trade Union of Fire Fighters
- Trade Union of Transport
- Trade Union of Workers in Science and Research
- Trade Union of Workers in Production and Specialised Organisations of Culture
- Trade Union of Employees in Aviation
- Trade Union of Workers of Cultural Facilities
- Trade Union of Workers of Culture and Nature Protection
- Trade Union of Employees in Libraries
- Actors’ Association
- UNIE - Trade Union of Professional Singers of the CR
- UNIE - Professional and Trade Union of Orchestral Musicians of the CR
- North-Bohemian Association of Trade Union Organisations in Mining Industry
- Rail Traffic Control Alliance
- Trade Union of Interpreters and Translators
- Media Trade Union